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Clinical Chemistry 19: 187-190, 1973;
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Clinical Chemistry, Vol 19, 187-190, Copyright © 1973 by the American Association for Clinical Chemistry

Radioimmunoassay of Plasma Digoxin, with Use of Iodinated Tracer

E. D. Horgan 1 and W. J. Riley 1

1 Department of Biochemistry, Royal Perth Hospital, Perth, Western Australia.

A radioimmunoassay of plasma digoxin is described, in which iodine-125 labeled digoxin is used as tracer. The assay is rapid, precise, sensitive, and specific, and can readily cope with a large number of plasma samples. The use of iodinated digoxin increases assay sensitivity, shortens counting time, and eliminates the problems associated with liquid scintillation counting. The performance of this method is compared with that of other assay systems involving the use of tritiated compounds.


Key Words: cardiac disorders, treatment • digitalis intoxication • dextran-coated charcoal • free and protein-bound digoxin

Submitted on August 28, 1972






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Copyright © 1973 by the American Association for Clinical Chemistry.