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Clinical Chemistry 19: 197-200, 1973;
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Clinical Chemistry, Vol 19, 197-200, Copyright © 1973 by the American Association for Clinical Chemistry

Overnight Radioimmunoassay of Human Follicle-Stimulating Hormone

Loretta P. Brown 1, Paige K. Besch 1, Dean S. Skelley 1, and Veasy C. Buttram Jr. 1

1 Department of Obstetrics and Gynecology, Baylor College of Medicine, Houston, Texas 77025; and the Reproductive Research Laboratory, St. Luke’s Episcopal Hospital, Houston, Texas 77025.

A radioimmunoassay procedure for follicle-stimulating hormone in serum has been developed in which the assay mixture is incubated at 37°C for a total of 22 h, rather than at 4°C for 4-7 days. The within-assay coefficients of variation for the 37°C and 4°C methods, respectively, are 7.7% and 5.0%, while the between-assay variations are 13.8% and 9.9%. Comparison of values for 4°C (x) were related to values for 37°C (y) according to the equation y = 1.2x + 0.8, with a correlation coefficient of 0.98.


Key Words: normal range • double-antibody system • diagnostic aid

Submitted on October 30, 1972
Accepted on November 22, 1972




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