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Clinical Chemistry 19: 201-204, 1973;
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Clinical Chemistry, Vol 19, 201-204, Copyright © 1973 by the American Association for Clinical Chemistry

Critical Variables in the Radioimmunological Technique for Measuring Immunoreactive Insulin with Use of Immunosorbents

Carlos A. Velasco 1, Werner Oppermann 1, and Rafael A. Camerini-Davalos 1

1 Diabetes Center, Department of Medicine, New York Medical College, New York, N.Y. 10029.

A commercial kit for determining immunoreactive insulin, the "Phadebas Insulin Test," was methodically evaluated to establish the optimal conditions for this immunoassay. Sensitivity, day-to-day and within-run precision, recovery, accuracy, and specificity are excellent. Results by this method compare favorably with those of the double-antibody technique. This rapid, simple, convenient, and reliable procedure for insulin immunoassay can be easily adapted to the facilities of a clinical laboratory as a standard diagnostic test or for clinical reseach.


Key Words: "Phadebas" test kit • double-antibody technique compared

Submitted on August 12, 1972
Accepted on November 29, 1972




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[Abstract] [PDF]


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Copyright © 1973 by the American Association for Clinical Chemistry.