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Clinical Chemistry 19: 205-209, 1973;
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Clinical Chemistry, Vol 19, 205-209, Copyright © 1973 by the American Association for Clinical Chemistry

Conditions for a Radioimmunoassay of Serum Parathyroid Hormone

Daisy S. McCann 1, JoAnn Prunty 1, Gerald Rigg 1, and Robert Arnstein 1

1 Department of Medicine, Wayne County General Hospital, Eloise, Mich. 48132, and the University of Michigan, Ann Arbor, Mich. (D. S. McC., J. P., and G. R.); and the Department of Medicine, Wayne State University, Detroit, Mich. (R. A.).

A radioimmunoassay for parathyroid hormone is described that circumvents some previous difficulties. Parathyroid hormone binds strongly to other serum components. Consequently, a "control" curve is set up for each specimen, the specimen consisting of serum, diluent, and radiolabel, but no antibody. Final calculations are then based on the difference between the control curve and the curve obtained by incubating serum, diluent, radiolabel, and antibody. The lack of parallelism between various sample curves makes it almost mandatory that curves derived from at least 12 dilutions of serum be included for each specimen.


Key Words: normal values • nonspecific binding of parathyroid hormone • radioiodination

Submitted on June 15, 1972
Accepted on November 30, 1972






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Copyright © 1973 by the American Association for Clinical Chemistry.