Clinical Chemistry
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Clinical Chemistry 19: 235-239, 1973;
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Clinical Chemistry, Vol 19, 235-239, Copyright © 1973 by the American Association for Clinical Chemistry

Polyacrylamide-Gel Block-Electrophoresis of Plasma Lipoproteins

Mitsuo Wada 1, Herbert K. Naito 1, L. A. Ehrhart 1, and Lena A. Lewis 1

1 Division of Research and the Division of Laboratory Medicine Cleveland Clinic Foundation, Cleveland, Ohio 44106.

We separated lipoprotein fractions on a preparative scale by two techniques of polyacrylamide-gel block electrophoresis. Completeness of separation of the various lipoproteins, which were subsequently extracted from the gels, was verified by re-electrophoresis and by immunologic techniques. A principal advantage of this method over ultracentrifugal isolation is the relatively short time required for the separation.

Submitted on November 17, 1972
Accepted on November 22, 1972






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Copyright © 1973 by the American Association for Clinical Chemistry.