Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 19: 308-311, 1973;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Meites, S.
Right arrow Articles by Saniel-Banrey, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Meites, S.
Right arrow Articles by Saniel-Banrey, K.

Clinical Chemistry, Vol 19, 308-311, Copyright © 1973 by the American Association for Clinical Chemistry

Modified Glucose Oxidase Method for Determination of Glucose in Whole Blood

Samuel Meites 1 and Karen Saniel-Banrey 1

1 Department of Pediatrics, The Ohio State University and The Children’s Hospital Research Foundation, 561 S. 17th St., Columbus, Ohio 43205.

We have modified an ultramicro method for determining glucose with glucose oxidase—peroxidase—odianisidine. Capillary blood is diluted in isotonic NaF (17.6-17.8 g/ liter), the cells are removed, and glucose is determined after a 20-min incubation with enzyme-containing reagent. Increasing the enzyme concentration fourfold nullifies interference from ascorbic acid and hemolysis, while greatly decreasing interference from bilirubinemia. If necessary, bilirubin is removed completely by coprecipitation with protein. Interference from uric acid is minimized by incorporating it into the standards. Several other suspected interferences proved inconsequential. Isotonic NaF does not inhibit the enzymes used, and preserves glucose in blood for at least 2 h. Avoiding protein precipitation and shortening the incubating time significantly lessens analytical time without affecting precision.


Key Words: glucose in plasma, serum, cerebrospinal fluid, urine • peroxidase • o-dianisidine • isotonic NaF diluent • pediatric application • predicting wholeblood glucose value from hematocrit, erythrocyte, plasma values

Submitted on November 16, 1972
Accepted on December 18, 1972






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1973 by the American Association for Clinical Chemistry.