Clinical Chemistry
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Clinical Chemistry 19: 312-314, 1973;
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Clinical Chemistry, Vol 19, 312-314, Copyright © 1973 by the American Association for Clinical Chemistry

Method for Determination of Cyclic AMP in Plasma

Babeth Rabinowitz 1 and Joseph Katz 1

1 Departments of Cardiology and Biochemistry, Cedars-Sinai Medical Center, Los Angeles, Calif. 90054.

A simple procedure is described for measuring cyclic AMP (cAMP) in plasma, body fluids, and tissue extracts. A modification of the method of Brown et al. [Biochem. J. 121, 561 (1971)], it is based on competitive binding of cAMP by a protein in crude adrenal extracts, and precipitation of the cAMP-protein complex by ammonium sulfate. cAMP concentrations in normal human plasma ranged from 8 to 16 nmol/liter, and in the plasma of dogs from 5 to 15 nmol/liter. The range of the method is 0.5-8 pmol per assay, and up to 0.4 ml of sample can be used. A cheap and easily prepared binding-protein is used and the procedure is suitable for clinical studies of cAMP concentrations in plasma.


Key Words: competitive preotein-binding • normal values. man and dog • precipitation of protein-bound cAMP with ammonium sulfate • radioactive cAMP

Submitted on October 2, 1972
Accepted on December 19, 1972






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Copyright © 1973 by the American Association for Clinical Chemistry.