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Clinical Chemistry 19: 338-340, 1973;
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Clinical Chemistry, Vol 19, 338-340, Copyright © 1973 by the American Association for Clinical Chemistry

Stable Reagents for Determination of Serum Triglycerides by a Colorimetric Hantzsch Condensation Method

Lowell B. Foster 1 and Ralph T. Dunn 1

1 Patterson Coleman Laboratories, 4600 N. Habana, Tampa, Fla. 33614.

We describe a modified method for determining triglycerides in serum, in which alumina is used to adsorb interfering substances, and the glycerol content of triglycerides is determined colorimetrically. In contrast to all previous Hantzsch condensation procedures in which periodate and acetylacetone are used, the working periodate and the working acetylacetone reagents are stable for at least six months because they are differently formulated. The principle that governs this increased stability applies to all Hantzsch condensation methods for triglycerides.


Key Words: Comparison of triglyceride methods • stable Hantzsch condensation reagents • direct colorimetric method

Submitted on October 13, 1972
Accepted on December 26, 1972




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