Clinical Chemistry
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Clinical Chemistry 19: 384-386, 1973;
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Clinical Chemistry, Vol 19, 384-386, Copyright © 1973 by the American Association for Clinical Chemistry

Turbidimetric Procedure for Determination of Lipase Activity

Angelo Burlina 1 and Lauro Galzigna 1

1 General Hospital of Conegliano, 31015 Conegliano, Italy; and the Department of Medical Biochemistry, University of Nairobi, P. O. Box 30197, Nairobi, Kenya.

A method is described for turbidimetric assay of lipase activity. The substrate is a two-phase emulsion composed of a fairly homogenous dispersion of micelles of either triolein or olive oil. The de-emulsification of the substrate after lipase addition is a true measure of the enzymatic activity. Turbidity change is related to amount of the enzyme by use of a commercial standard, purified lipase from hog pancreas. The quantitative relationship between turbidity change and the number of micelles provides the theoretical basis for this type of assay.


Key Words: triolein or olive oil as substrates • porcine lipase as standard • normal values

Accepted on January 4, 1973






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Copyright © 1973 by the American Association for Clinical Chemistry.