Clinical Chemistry
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Clinical Chemistry 19: 403-407, 1973;
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Clinical Chemistry, Vol 19, 403-407, Copyright © 1973 by the American Association for Clinical Chemistry

Nephelometric Studies of the Precipitin Reaction: A Model System for Specific Protein Measurements

L. M. Killingsworth 1 and John Savory 1

1 Department of Pathology, University of Florida College of Medicine, Gainesville, Fla. 32601; and the Departments of Medicine, Biochemistry, and Pathology, University of North Carolina, Chapel Hill, N. C. 27514.

Light-scattering studies have been carried out on an immunochemical system that serves as a model for specific protein determinations in biological samples. The influence of antigen/antibody ratio, salts, urea, pH, temperature, and excitation wavelength are presented. Of significance is the finding that ions, particularly F-, increase the rate of formation of and the light scattering from the antigen-antibody complexes.


Key Words: IgG-anti IgG system • factors affecting light-scattering by antigen—antibody complexes

Submitted on November 28, 1972
Accepted on January 12, 1973






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Copyright © 1973 by the American Association for Clinical Chemistry.