Clinical Chemistry
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Clinical Chemistry 19: 411-414, 1973;
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Clinical Chemistry, Vol 19, 411-414, Copyright © 1973 by the American Association for Clinical Chemistry

Comparison of the Stannous Chloride and Vanadate Methods for Estimation of Serum Inorganic Phosphorus by Use of the "SMA 12/60"

Janet L. Davies 1, G. S. Andrews 1, R. Miller 1, and H. G. Owen 1

1 Department of Biochemistry, The Royal Gwent Hospital, Newport, Monmouthshire, U. K.

We compared results of the stannous chloride and vanadate methods for estimating serum inorganic phosphorus on the "SMA 12/60" (Technicon). The technically simpler vanadate method gave better linearity and reproducibility, with virtually no carry-over or baseline drift; the reagents were stable, and it was most important to ensure that the concentration of sulfuric acid in the recipient and sample streams was identical. Instability in the stannous chloride method was due to precipitation in the stannous chloride-hydrazine working solution.

Submitted on November 22, 1972
Accepted on January 29, 1973






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Copyright © 1973 by the American Association for Clinical Chemistry.