Clinical Chemistry
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Clinical Chemistry 19: 419-424, 1973;
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Clinical Chemistry, Vol 19, 419-424, Copyright © 1973 by the American Association for Clinical Chemistry

Colorimetric Microdetermination of Free Fatty Acids

Felix G. Soloni 1 and Laura C. Sardina 1

1 Chemistry Research and Development Laboratories, Dade Division, American Hospital Supply Corp., Miami, Fla. 33152.

A colorimetric method for determination of free fatty acids has been developed, based on the estimation of copper in a chloroform extract of their cupric salts with oxalic acid bis-(cyclohexylidenehydrazide). Contamination with inorganic copper during extraction is practically eliminated by using filter-paper pads. Specificity and interferences are discussed. Recoveries average 101.1%. Normal values are 10.0 ± 8.2 mg/dl (0.390 ± 0.320 mmol/liter), with no statistically significant sex-related difference. Correlation with the reference method of Dole and Meinertz [J. Biol. Chem. 235, 2595 (1960)] is 0.954. Ten samples can be measured in about 45 min.


Key Words: cuprizone reagent used with copper salts of free fatty acids • normal values

Submitted on November 1, 1972
Accepted on January 29, 1973






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Copyright © 1973 by the American Association for Clinical Chemistry.