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Clinical Chemistry 19: 425-428, 1973;
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Clinical Chemistry, Vol 19, 425-428, Copyright © 1973 by the American Association for Clinical Chemistry

Reduction of Glyoxylate by Human Serum

G. Young 1, F. Naghizadeh 1, J. King 1, and H. G. Morgan 1

1 Department of Biochemistry and University Department of Pathological Biochemistry, Royal Infirmary, Glasgow, U.K.

Glyoxylate is enzymically reduced by human serum. Optimal concentrations of hydrogen ion, substrate, and coenzyme for this reduction by sera from healthy persons and from patients with disease of the heart or liver have been determined at 37°C. At this temperature, the reaction proceeds faster than with pyruvate or 2-oxobutyrate but more slowly than with hydroxypyruvate. Lactate dehydrogenase appears to be the enzyme involved.


Key Words: lactate dehydrogenase • isoenzymes • "glycollate dehydrogenase" • glyoxylate reductase

Submitted on December 21, 1972
Accepted on January 29, 1973






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Copyright © 1973 by the American Association for Clinical Chemistry.