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Clinical Chemistry 19: 453-458, 1973;
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Clinical Chemistry, Vol 19, 453-458, Copyright © 1973 by the American Association for Clinical Chemistry

An Approach to the Measurement of Total Lipid Glycerol in Serum

Donald L. Horney 1

1 From the Highland Park Hospital, Highland Park, III. 60035.

Serum phospholipids that derive from glycerol are hydrolyzed to diglycerides and by-products in the presence of phospholipase C (phosphatidylcholine cholinephosphohydrolase, EC 3.1.4.3). The diglycerides produced are saponified, together with the triglycerides present, to glycerol and salts of fatty acids. Measuring the glycerol produced yields a measure of the total lipid glycerol in serum, and is useful for the estimation of the phospholipid and total lipid content of serum.


Key Words: phospholipase C • phospholipids • use of "kits" for glycerol assay and phosphorus assay

Submitted on August 10, 1972
Accepted on February 5, 1973






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Copyright © 1973 by the American Association for Clinical Chemistry.