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Clinical Chemistry 19: 506-510, 1973;
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Clinical Chemistry, Vol 19, 506-510, Copyright © 1973 by the American Association for Clinical Chemistry

A Modified Indirect Method for Determining Erythrocyte Sodium and Potassium Concentrations

Larry L. Small 1 and Dwight B. Coulter 1

1 From the Department of Physiology and Pharmacology, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50010.

An automated method was adapted to measure the concentration of Na and K in plasma, nonhemolyzed whole blood, and hemolyzed whole blood, and thus allow the calculation of erythrocyte electrolyte concentrations by a modified indirect method. The Na concentrations of nonhemolyzed whole blood and plasma were used to calculate the percent cell volume (hematocrit) of a blood sample. The percent cell volume and concentrations of Na and K in nonhemolyzed whole blood were used to modify the indirect method of calculating erythrocyte Na and K concentrations in canine, porcine, and human blood samples. Significant differences were found between the two indirect methods (indirect and modified indirect) used to calculate erythrocyte Na and K concentrations of human blood samples.


Key Words: packed cell volume • hematocrit • erythrocyte electrolytes • trapped plasma • dilution-dialysis • AutoAnalyzer • data from pigs, dogs • Na cell volume

Submitted on January 9, 1973
Accepted on February 16, 1973






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Copyright © 1973 by the American Association for Clinical Chemistry.