Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 19: 511-515, 1973;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ficher, M.
Right arrow Articles by Perry, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ficher, M.
Right arrow Articles by Perry, S.

Clinical Chemistry, Vol 19, 511-515, Copyright © 1973 by the American Association for Clinical Chemistry

Improved Measurement of Corticosteroids in Plasma and Urine by Competitive Protein-Binding Radioassay

Miguel Ficher 1, George C. Curtis 1, V. K. Ganjam 1, Leon Joshlin 1, and Sarah Perry 1

1 Department of Pediatrics (St. Christopher’s Hospital for Children), Temple University School of Medicine, Philadelphia, Penna. 19133; the Eastern Pennsylvania Psychiatric Institute, Philadelphia, Penna. 19129; and the New Bolton Center, University of Pennsylvania School of Veterinary Medicine, Kennett Square, Penna. 19348.

In the assay of corticosteroids in plasma and urine by competitive protein binding, the use of horse serum or plasma as a source of assay protein gives better sensitivity and somewhat better specificity for cortisol than do previously described procedures. Five to 10 µl of plasma unknown or 50 to 100 µl of urine unknown can be used. Precision for the standard curve is 0.12 ng in the range 0-2.00 ng, and accuracy, measured as the amount of added cortisol recovered from plasma, is about 91%. Because of the improved specificity, the need for preliminary purification by chromatography is decreased for some purposes. The same corticosteroid-binding globulin and standard curve can be used for assaying corticosteroids in urine or plasma. The procedure may be useful for unusually small samples or if discriminations within the lower range of physiological concentrations are needed, as in work with infants and neonates or in the study of small adrenal secretory pulses, such as those occurring at the nadir of the circadian cycle.


Key Words: horse serum vs. dog or human serum as source of assay protein • corticosteroid-binding globulin, specificity • radiolabeled steroids • small sample requirement

Submitted on January 26, 1973
Accepted on March 8, 1973






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1973 by the American Association for Clinical Chemistry.