Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 19: 522-524, 1973;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kabasakalian, P.
Right arrow Articles by Westcott, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kabasakalian, P.
Right arrow Articles by Westcott, A.

Clinical Chemistry, Vol 19, 522-524, Copyright © 1973 by the American Association for Clinical Chemistry

Determination of Uric Acid in Serum, with Use of Uricase and a Tribromophenol—Aminoantipyrine Chromogen

Peter Kabasakalian 1, Sami Kalliney 1, and Anita Westcott 1

1 Research Division, Schering Corp., Bloomfield, N. J. 07003.

A manual method is described for determining the hydrogen peroxide produced from uric acid with uricase (pH 9.2) after deproteinization of 1.00 ml of serum. In the method, 2,4,6-tribromophenol is coupled with 4-aminoantipyrine (pH 7.0) by peroxidase oxidative coupling. The sensitivity of the method is such that 25 µg of uric acid from a fourth of the deproteinized serum (100 mg/liter) in a final volume of 5 ml of n-butyl acetate color extract gives a stable absorbance of 0.7 at 492 nm with a 1-cm cell. The method is reproducible, and many substances that may be encountered in serum do not interfere. Absorbances are linear for uric acid concentrations as high as 200 mg/liter.

Submitted on January 17, 1973
Accepted on February 12, 1973






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1973 by the American Association for Clinical Chemistry.