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Clinical Chemistry 19: 526-528, 1973;
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Clinical Chemistry, Vol 19, 526-528, Copyright © 1973 by the American Association for Clinical Chemistry

An Automated Procedure, with Use of Ferrozine, for Assay of Serum Iron and Total Iron-Binding Capacity

J. M. White 1 and H. A. Flashka 1

1 Laboratory Division, Clinical Chemistry and Hematology Branch, Center for Disease Control, Atlanta, Ga. 30333; and the Chemistry Department, Georgia Institute of Technology, Atlanta, Ga.

In this procedure of analysis for iron in serum and measurement of iron-binding capacity, the Beckman Model 560 DSA and ferrozine are used. The analysis rate is 120 samples per hour. Interferences were investigated and a procedure was devised for masking high concentrations of copper. A modified procedure was used to prepare samples for the assay of total iron-binding capacity. This procedure is more rapid and requires smaller amounts of sample than many methods now in use.


Key Words: Beckman DSA

Submitted on October 27, 1972
Accepted on February 16, 1973







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Copyright © 1973 by the American Association for Clinical Chemistry.