Clinical Chemistry
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Clinical Chemistry 19: 529-530, 1973;
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Clinical Chemistry, Vol 19, 529-530, Copyright © 1973 by the American Association for Clinical Chemistry

Stability of Erythrocytic Enzymes for Screening Tests

Mei L. Lowe 1, Jerry B. Gin 1, and James A. Demetriou 1

1 Research Department, Bio-Science Laboratories, 7600 Tyrone Ave., Van Nuys, Calif. 91405.

We investigated the stability of certain erythrocytic enzymes as assayed qualitatively by fluorescence spot tests. Preservation of the activities of glucose-6-phosphate dehydrogenase (GPD), pyruvate kinase (PK), triose phosphate isomerase (TPI), glutathione reductase (GTD), and NADH diaphorase (ND) in heparin, glucose—EDTA, or Alsever’s solution was studied at -20°C and 30°C. In Alsever’s solution, GPD, PK, GTD, and TPI are stable for 25 days at 30°C, whereas ND is stable for only 10 days at either -20°C or 30°C. The stabilizing action of Alsever’s solution permits either large population sampling before testing or a sufficient time period for transport of samples to a central laboratory for processing.


Key Words: Alsever’s solution • glucose-EDTA • heparin • storage temperatures

Submitted on February 2, 1973
Accepted on February 26, 1973






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Copyright © 1973 by the American Association for Clinical Chemistry.