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Clinical Chemistry 19: 602-607, 1973;
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Clinical Chemistry, Vol 19, 602-607, Copyright © 1973 by the American Association for Clinical Chemistry

Radioimmunoassay of Human Chorionic Somatomammotropin in Serum, Amniotic Fluid, and Urine

Russell J. Moser 1 and Dorothy R. Hollingsworth 1

1 Department of Pediatrics, College of Medicine, University of Kentucky, Lexington, Ky. 40506.

A method is described for measurement of human chorionic somatomammotropin (HCS) by use of a double-antibody technique, for concentrations ranging from 2 ng/ml to 20 µg/ml. The cross-reactive characteristics of rabbit anti-human HCS serum No. CT 3399 from NIAMD are explored. A dilution plan is presented utilizing a standard curve from 0.75 to 80 ng/ml. Concentrations (mean and ranges) for HCS in maternal serum (evaluated every four weeks during pregnancy), umbilical cord serum, serum from babies, amniotic fluid, and urine are given.


Key Words: double-antibody technique • rabbit anti-human HCS serum No. CT 3399 from NIAMD • HCS concentrations in pregnancy serum (evaluated every four weeks), in serum of babies, in urine (by trimester), in amniotic fluid, and in serum from umbilical cord blood

Submitted on January 15, 1973
Accepted on April 5, 1973






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Copyright © 1973 by the American Association for Clinical Chemistry.