Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 19: 621-623, 1973;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Van Kley, H.
Right arrow Articles by Claywell, C. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Van Kley, H.
Right arrow Articles by Claywell, C. S.

Clinical Chemistry, Vol 19, 621-623, Copyright © 1973 by the American Association for Clinical Chemistry

Evaluation of EDTA as the Chelator in the Biuret Reagent

Harold Van Kley 1 and Carol S. Claywell 1

1 Laboratory for Biochemical Research, St. Mary’s Health Center, 6420 Clayton Rd., St. Louis, Mo. 63117; and the Department of Biochemistry, St. Louis University School of Medicine, St. Louis, Mo.

Use of ethylenediaminetetraacetate (EDTA) as the chelating agent in the biuret reaction (in place of tartrate or citrate) was investigated. We found that this suggested modification of the reagent results in lower color yields and prolongs the time for color development. The EDTA reagent is more stable if color development is hastened by heating, but conditions are described under which the more customary reagent may be so used.

Submitted on February 28, 1973
Accepted on April 6, 1973






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1973 by the American Association for Clinical Chemistry.