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Clinical Chemistry 19: 623-625, 1973;
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Clinical Chemistry, Vol 19, 623-625, Copyright © 1973 by the American Association for Clinical Chemistry

Evaluation of an Automated Creatininase Creatinine Procedure

Monty H. McLean 1, Jerry Gallwas 1, and Mark Hendrixson 1

1 Applications Research, Clinical Instruments Division, Beckman Instruments, Inc., 2500 Harbor Blvd., Fullerton, Calif. 92634.

We have evaluated an automated method for determining serum creatinine by use of the enzyme creatininase. The method has been adapted to the Beckman DSA-560 and measures the Jaffé-positive chromogens present in serum before and after enzymatic hydrolysis of creatinine. The precision and linearity of the method are acceptable, and the results correlate well with those obtained by other accepted techniques. The method offers the advantages of automation, enhanced specificity, micro-scale sample volume, and direct serum analysis.

Submitted on February 1, 1973
Accepted on March 16, 1973






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Copyright © 1973 by the American Association for Clinical Chemistry.