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Clinical Chemistry 19: 737-739, 1973;
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Clinical Chemistry, Vol 19, 737-739, Copyright © 1973 by the American Association for Clinical Chemistry

Improved Techniques for Assessment of Plasma Lipoprotein Patterns. I. Precipitation in Gels after Electrophoresis with Polyanionic Compounds

Dietrich Seidel 1, Heinrich Wieland 1, and Claudia Ruppert 1

1 Medizinische Universitätsklinik (Ludolf-Krehl-Klinik), Bergheimer Strasse 58, D-69 Heidelberg, FRG.

A method is described for making lipoprotein fractions visible by polyanion precipitation in situ in the gels after electrophoresis. With the new technique the pattern and its interpretation in the differential diagnosis of all primary forms of hyperlipoproteinemia is the same as for the other lipid-staining procedures. The new technique is simpler to perform, reliable, and provides the required information 60 min after electrophoresis. It also allows a very fast determination of the abnormal lipoprotein (LP-X) that characterizes cholestasis, without use of a specific antiserum.


Key Words: electrophoresis in agarose, in agar gel, in polyacrylamide • serum lipoprotein pattern • hyperlipoproteinemia • Ca-dextran sulfate as lipoprotein precipitant • immunodiffusion • LP-X • diagnosis of cholestasis

Submitted on February 7, 1973
Accepted on April 30, 1973




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Copyright © 1973 by the American Association for Clinical Chemistry.