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Clinical Chemistry 19: 817-820, 1973;
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Clinical Chemistry, Vol 19, 817-820, Copyright © 1973 by the American Association for Clinical Chemistry

Sensitive Fluorescence Monitoring of Aromatic Acids after Anion—Exchange Chromatography of Body Fluids

S. Katz 1, W. W. Pitt Jr. 1, and G. Jones Jr. 1

1 Oak Ridge National Laboratory,1 Oak Ridge, Tenn. 37830.

A simple, sensitive system has been developed for detecting oxidizable compounds that are eluted from chromatographic columns. It relies upon the production of fluorescent cerium(III) by the reaction of eluted compounds with cerium(IV) solution. When coupled with a modified ultraviolet-monitored anion-exchange chromatograph, this system provides excellent resolution and sensitivity in the analytical determination of aromatic organic acids.

Submitted on March 23, 1973
Accepted on April 4, 1973






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Copyright © 1973 by the American Association for Clinical Chemistry.