Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 19: 821-825, 1973;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schneider, R. S.
Right arrow Articles by Ullman, E. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schneider, R. S.
Right arrow Articles by Ullman, E. F.

Clinical Chemistry, Vol 19, 821-825, Copyright © 1973 by the American Association for Clinical Chemistry

Homogeneous Enzyme Immunoassay for Opiates in Urine

Richard S. Schneider 1, Peggy Lindquist 1, Emmy Tong-in Wong 1, Kenneth E. Rubenstein 1, and Edwin F. Ullman 1

1 Syva Research Institute, 3221 Porter Drive, Palo Alto, Calif. 94304.

An assay technique, homogeneous enzyme immunoassay, is described for the quantitative determination of morphine derivatives in biological fluids. An enzyme is labeled with morphine. When the enzyme-labeled morphine is bound by antimorphine antibodies, the enzyme is rendered inactive. Free morphine competes with enzyme—morphine for antibody binding sites preventing inhibition of enzyme activity. Enzyme activity is thus directly related to the concentration of free morphine.

See PDF for Equation

Where the enzyme used is lysozyme, the assay can detect 0.5 µg of morphine per milliliter of urine with >95% confidence (CV, 5.0% at 0.5 µg/ml morphine). The technique gave nearly twice as many confirmed positives as did thin-layer chromatography in a comparative study of urines from a methadone maintenance program. The immediacy of the results (assay time: <1 min) permits use of the assay in hospital emergency rooms, law-enforcement facilities, and methadone treatment programs.


Key Words: "EMIT" system • speedy detection of drug abuse • results by radioimmunoassay and thin-layer chromatography compared

Submitted on June 11, 1973




The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
R. Rej
Clinical Chemistry through Clinical Chemistry: A Journal Timeline
Clin. Chem., December 1, 2004; 50(12): 2415 - 2458.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
A. Ruttenber and J. Luke
Heroin-related deaths: new epidemiologic insights
Science, October 5, 1984; 226(4670): 14 - 20.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1973 by the American Association for Clinical Chemistry.