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Clinical Chemistry 19: 826-831, 1973;
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Clinical Chemistry, Vol 19, 826-831, Copyright © 1973 by the American Association for Clinical Chemistry

Computer Technique for Identification of Chromatographic Peaks

D. D. Chilcote 1

1 Oak Ridge National Laboratory,1 Oak Ridge, Tenn. 37830.

A computer program has been developed to identify known peaks in a chromatogram from a computer-interfaced chromatograph. The focal point of the program is a normalization technique, which refers the elution volume of each sample peak encountered to the elution volumes of two reference peaks that bracket the peak of interest, as is done in certain gas chromatographic normalization techniques. The normalized elution volume is determined by dividing the difference between the elution volumes of the sample peak and the first (early-eluting) reference peak by the difference between the elution volumes of the two reference peaks. The normalized elution volumes thus obtained are compared to normalized elution volumes of known compounds stored in bulk memory. The compounds now being used as reference peaks in the chromatograms from the UV-Analyzer are hypoxanthine, uric acid, hippuric acid, and p-cresol.


Key Words: column chromatography of biological fluids • normalization of elution volumes

Submitted on April 16, 1973
Accepted on May 19, 1973




The following articles in journals at HighWire Press have cited this article:


Home page
 ScienceHome page
C. D. Scott
High-Pressure Ion Exchange Chromatography
Science, October 18, 1974; 186(4160): 226 - 233.
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Copyright © 1973 by the American Association for Clinical Chemistry.