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Clinical Chemistry 19: 838-844, 1973;
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Clinical Chemistry, Vol 19, 838-844, Copyright © 1973 by the American Association for Clinical Chemistry

Design, Construction, and Two Applications for an Automated Flow—Cell Polarization Fluorometer with Digital Read Out: Enzyme-Inhibitor (Antitrypsin) Assay and Antigen—Antibody (Insulin—Insulin Antiserum) Assay

Richard D. Spencer 1, Fernando B. Toledo 1, Ben T. Williams 1, and Norma L. Yoss 1

1 Mercy Hospital Laboratory, Urbana, Ill. 61801; and the School of Chemical Sciences, Department of Biochemistry, University of Illinois, Urbana, Ill. 61801 (R.D.S.)

We have constructed and automated a flow cell polarization fluorometer and demonstrated two specific clinical applications of fluorescence polarization assay, i.e., the enzyme-inhibitor assay of antitrypsin in serum and the antigen-antibody assay of insulin and its antibody. A signal is displayed directly and is immediately available for chart recording and (or) digital data processing. Since fluorescence polarization offers a number of choices in assay parameters and the use of different fluorescent probes, its development for rapid simultaneous measurements of multiple components in body fluids should be possible.


Key Words: mass screening • pulmonary emphysema • infantile cirrhosis • diagnostic aids • normal

Submitted on May 16, 1973
Accepted on June 11, 1973




The following articles in journals at HighWire Press have cited this article:


Home page
 J Biomol ScreenHome page
M. E. Jolley
Fluorescence Polarization Assays for the Detection of Proteases and Their Inhibitors
J Biomol Screen, February 1, 1996; 1(1): 33 - 38.
[Abstract] [PDF]


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Copyright © 1973 by the American Association for Clinical Chemistry.