Clinical Chemistry
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Clinical Chemistry 19: 919-922, 1973;
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Clinical Chemistry, Vol 19, 919-922, Copyright © 1973 by the American Association for Clinical Chemistry

Use of High-Pressure Liquid Chromatography for Monitoring Nucleotide Concentration in Human Blood: A Preliminary Study with Stored Blood Cell Suspensions

Phyllis R. Brown 1, R. E. Parks Jr. 1, and Jane Herod 1

1 Section of Biochemical Pharmacology, Division of Biological and Medical Sciences, Brown University, Providence, R.I. 02912.

The development of high-pressure liquid chromatography has made available a new technique for monitoring nucleotide concentrations in biological materials sensitively, accurately, and rapidly. Because there appears to be a correlation between ATP concentrations and viability of stored blood, a preliminary study was carried out on blood cell suspensions to evaluate the technique for possible use in blood banks, clinical laboratories, and research on preservatives for blood.


Key Words: viability of stored blood • blood banking • ATP, ADP, AMP • blood preservation

Submitted on April 4, 1973
Accepted on May 15, 1973






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Copyright © 1973 by the American Association for Clinical Chemistry.