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Clinical Chemistry 19: 923-926, 1973;
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Clinical Chemistry, Vol 19, 923-926, Copyright © 1973 by the American Association for Clinical Chemistry

Measurement of Putrescine, Spermidine, and Spermine in Physiological Fluids by Use of an Amino Acid Analyzer

Laurence J. Marton 1, Diane H. Russell 1, and Carl C. Levy 1

1 Baltimore Cancer Research Center, National Cancer Institute, Baltimore, Md. 21211.

Elevated polyamine concentrations in human urine and serum appear to be associated with the presence of many types of cancer. The automated amino acid analyzer lends itself to the rapid separation and measurement of these substances with little sample handling and excellent reproducibility. The ion-exchange resin used was Beckman’s PA-35 (strong sulfonic acid cation exchanger, copolymerization product of styrene-divinylbenzene). Amino acids are bound less strongly to the resin than are the polyamines, and can be removed by first eluting with a buffer of lower ionic strength. If sample injections are overlapped, one sample can be analyzed every hour. We have corroborated that increased concentrations of polyamines appear in the urine of patients with a variety of cancers. Additionally, we have shown increased amounts of polyamines in the serum of patients having a diagnosis of cancer.


Key Words: cancer diagnosis • polyamines

Submitted on February 28, 1973
Accepted on April 16, 1973




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Copyright © 1973 by the American Association for Clinical Chemistry.