Clinical Chemistry
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Clinical Chemistry 19: 1010-1015, 1973;
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Clinical Chemistry, Vol 19, 1010-1015, Copyright © 1973 by the American Association for Clinical Chemistry

Spectrophotometric Quantification of Glycine in Serum and Urine

Jesse F. Goodwin 1 and Suresh Stampwala 1

1 General Clinical Research Center for Children, Wayne State University School of Medicine and Children’s Hospital of Michigan, Detroit, Mich. 48201.

A method for quantitative determination of glycine in serum and urine, involving degradation to formaldehyde with chloramine-T and subsequent measurement by the Hantzsch reaction or chromotropic acid, has been evaluated and modified. The inhibitory effect of some physiological constituents on the analysis is pointed out. We recommend that samples be purified by passage through a column of ion-exchange resin before analysis. Results for urinary glycine obtained by a single-column amino acid analyzer system compare very favorably with those obtained by the proposed spectrophotometric method. Values for free and total glycine and for glycine/creatinine ratios in the urine of a group of infants are presented along with revised data on the concentration of free glycine in serum.


Key Words: Hantzsch reaction • chromotropic acid reagent • 3,5-diacetyl-1, 4-dihydrolutidine formation • agreement with column-chromatographic results • hyperglycinemia • normal values for infants

Submitted on February 22, 1973
Accepted on June 18, 1973






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Copyright © 1973 by the American Association for Clinical Chemistry.