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Clinical Chemistry 19: 1022-1026, 1973;
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Clinical Chemistry, Vol 19, 1022-1026, Copyright © 1973 by the American Association for Clinical Chemistry

Automated Method for Serum Glucose with p-Hydroxybenzoic Acid Hydrazide

Benjamin Fingerhut 1

1 Institute of Pathology, Kings County Hospital Center, Brooklyn, N. Y. 11203.

A new automated method for serum glucose is presented, based on the reaction between glucose and p-hydroxybenzoic acid hydrazide in alkaline solution. Results for 70 non-uremic sera averaged 60 mg/liter greater than those given by an automated glucose oxidase (EC 1.1.3.4) procedure. Values for uremic sera indicated the proposed method to be more specific for such sera than is the automated ferricyanide-reduction technique. Within-run and day-to-day values had a standard deviation of 17 and 32 mg/ liter, respectively. Recoveries ranged from 99.5 to 104.5% (mean, 102.2%).


Key Words: methods for glucose intercompared • AutoAnalyzer

Submitted on February 28, 1973
Accepted on June 18, 1973






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Copyright © 1973 by the American Association for Clinical Chemistry.