Clinical Chemistry
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Clinical Chemistry 19: 1031-1035, 1973;
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Clinical Chemistry, Vol 19, 1031-1035, Copyright © 1973 by the American Association for Clinical Chemistry

Determination of 3-Methoxy-4-Hydroxyphenylethylene Glycol, a Noradrenaline Metabolite, in Cerebrospinal Fluid and Urine

R. O’Keeffe 1 and B. W. L. Brooksbank 1

1 MRC Neuropsychiatry Unit, West Park Hospital, Epsom, Surrey, U.K.

We describe a sensitive, accurate method for quantitative determination of 3-methoxy-4-hydroxyphenylethylene glycol (MHPG) in cerebrospinal fluid and urine. The method entails enzymatic hydrolysis of conjugated MHPG, acetylation of the phenolic group, thin-layer chromatographic purification, and estimation of total MHPG as the acetyl heptafluorobutyryl derivative by gas—liquid chromatography with electron capture detection and with use of aldrin as internal standard. Results obtained by this method compare favorably with those by other published procedures. Precision, calculated as the mean ± SD of the percentage differences between duplicate estimations, was 8.4 ± 8.0% for cerebrospinal fluid (3 ml) or 6.9 ± 6.6% for urine (2 ml). Total MHPG in lumbar cerebrospinal fluid specimens from 49 individuals ranged from 6.1 to 19.7 ng/ml, with no significant difference between the mean for control subjects and that for patients with affective disorders.


Key Words: free and conjugated MHPG • noradrenaline metabolism • thin-layer chromatography • gas—liquid chromatography with electron-capture detection • normal values • MHPG in ventricular and cisternal fluid • affective disorders

Submitted on April 6, 1973
Accepted on June 19, 1973




The following articles in journals at HighWire Press have cited this article:


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T. Keeton, H Krutzsch, and W Lovenberg
Specific and sensitive radioimmunoassay for 3-methoxy-4-hydroxyphenylethyleneglycol (MOPEG)
Science, February 6, 1981; 211(4482): 586 - 588.
[Abstract] [PDF]


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Copyright © 1973 by the American Association for Clinical Chemistry.