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Clinical Chemistry 19: 1045-1048, 1973;
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Clinical Chemistry, Vol 19, 1045-1048, Copyright © 1973 by the American Association for Clinical Chemistry

A "Reagentless" Fluorometric Method for Creatine Kinase Activity in Serum

Herbert K. Y. Lau 1 and George G. Guilbault 1

1 Department of Chemistry, Louisiana State University in New Orleans, New Orleans, La. 70122.

A "reagentless" fluorometric method is described for the analysis of serum creatine kinase (CK) activity. The method is based on the use of silicone rubber pads, upon which are placed all the reagents for assay of CK. The rate of formation of NADH fluorescence at 460 nm is measured and equated to CK activity. The method is simple, rapid, inexpensive, and as little as 3 µl of serum is needed.


Key Words: reagents impregnated onto silicone rubber pads

Submitted on May 16, 1973
Accepted on June 20, 1973






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Copyright © 1973 by the American Association for Clinical Chemistry.