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Clinical Chemistry 19: 1079-1080, 1973;
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Clinical Chemistry, Vol 19, 1079-1080, Copyright © 1973 by the American Association for Clinical Chemistry

Effect of Various Diluents on the Activity of Several Enzymes Present in Serum

Ted W. Fendley 1, Jane M. Hochholzer 1, and Christopher S. Frings 1

1 Medical Laboratory Associates, 1025 S. 18th St., Birmingham, Ala. 35205.

We have evaluated the effect of diluting serum with water or NaCl solution (8.5 or 9.0 g/liter) before assaying by a manual method for creatine kinase (EC 2.7.3.2), alkaline phosphatase (EC 3.1.3.1), lactate dehydrogenase (EC 1.1.1.27), and aspartate aminotransferase (EC 2.6.1.1) activity. The t test and the F test show no significant difference in the accuracy and precision of the assays at the 95% confidence level when 100 different samples were compared for each enzyme activity after use of the three diluents.

Submitted on May 4, 1973
Accepted on June 23, 1973






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Copyright © 1973 by the American Association for Clinical Chemistry.