Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 19: 973-976, 1973;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Laessig, R. H.
Right arrow Articles by Paskey, T. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Laessig, R. H.
Right arrow Articles by Paskey, T. A.

Clinical Chemistry, Vol 19, 973-976, Copyright © 1973 by the American Association for Clinical Chemistry

Evaluation and Recalculation of Manufacturer’s Instrument-Specific Aspartate Aminotransferase Values for Reference Sera

Ronald H. Laessig 1, Andris Indriksons 1, Thomas H. Schwartz 1, and Toni A. Paskey 1

1 State Laboratory of Hygiene University of Wisconsin Medical Center, Madison, Wis. 53706; and the Multiphasic Health Screening Program, Department of Health and Social Services, Madison, Wis. 53706.

We compared a manufacturer’s stated values to those obtained by a reference method for aspartate aminotransferase activity in reference sera. We determined the validity with which this manufacturer’s stated values may be used as multichannel-analyzer set points, and related values obtained by the reference method to instrument-specific values by regression analysis. Data were obtained from 40 multichannel analyzers, comprising three different instruments (and their respective procedures): SMA 12/ 60, ultraviolet and colorimetric; SMA 12/30, colorimetric (reported in Karmen units); and MARK X, colorimetric. Results that best correspond with those for the Karmen-uv reference method are those obtained by automated methods that most closely emulate it. Regression equations relate Karmen (reference method) results to instrument-specific values. These relationships enable laboratories to check assay values of their lots of reference or control sera against a consensus value obtained from the group of similar instruments used in the study. Regression equations are calculated for the intercomparison of results from the four automated procedures.


Key Words: results from automated instrumentation intercompared • Hycel MARK X • SMA 12/30 and 12/60 • colorimetric and uv methods for aspartate aminotransferase • method of Karmen

Submitted on December 28, 1972
Accepted on June 4, 1973






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1973 by the American Association for Clinical Chemistry.