Clinical Chemistry
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Clinical Chemistry 19: 984-993, 1973;
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Clinical Chemistry, Vol 19, 984-993, Copyright © 1973 by the American Association for Clinical Chemistry

Standardization in Bilirubin Assays: Evaluation of Selected Methods and Stability of Bilirubin Solutions

Basil T. Doumas 1, Billy Wayne Perry 1, Edward A. Sasse 1, and Jon V. Straumfjord Jr. 1

1 Department of Pathology, The Medical College of Wisconsin, 8700 West Wisconsin Ave., Milwaukee, Wis. 53226.

Because of the inaccuracy of bilirubin (BIL) determinations, we have investigated some sources of error associated with the most commonly used methods. Inadequate standardization appears to be the most common error. BIL standards in either human serum albumin (HSA), bovine serum albumin (BSA), or pooled human serum were analyzed by these methods. Molar absorptivities (egr) of azobilirubin by the Jendrassik—Gróf procedure were practically identical in all three protein bases. The Meites—Hogg and Malloy—Evelyn methods gave substantially higher egr values with BIL in serum than with BIL in either HSA or BSA. The precision of all methods was good, but best with the Jendrassik—Gróf procedure. BIL standards can be prepared with good reproducibility (CV <0.5%). Standards deteriorate appreciably at -23 °C but are stable at -70 °C. Our data indicate a need for improved commercial BIL controls.


Key Words: sources of error in bilirubin determination • inter-method comparison • stability of bilirubin • bilirubin standards

Submitted on April 5, 1973
Accepted on June 5, 1973




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Copyright © 1973 by the American Association for Clinical Chemistry.