Clinical Chemistry Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 2: 99-111, 1956;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Laurell, C. B.
Right arrow Articles by Skoog, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Laurell, C. B.
Right arrow Articles by Skoog, N.

Clinical Chemistry, Vol 2, 99-111, Copyright © 1956 by the American Association for Clinical Chemistry

Buffer Composition in Paper Electrophoresis

Considerations on its Influence, with Special Reference to the Interaction Between Small Ions and Proteins

C. B. Laurell 1, S. Laurell 1, and Nancy Skoog 1

1 Laboratories of Clinical Chemistry at the Hospitals of Malmö and Lund, Sweden.

An apparatus is described for paper electrophoresis according to the moist-chamber principle. A simple method for quantitative evaluation of the different electrophoretic fractions is presented together with the limits for the normal variation of the different serum fractions.

The influence of the composition of the buffer on the separation of the proteins is stressed.

Addition of small amounts of calcium ions results in a separation of the beta-fraction into two distinct fractions, beta1 and beta2Ca. The mobility of beta-lipoproteins is decreased in the presence of calcium. The phenomenon was confirmed with the aid of moving-boundary electrophoresis. The refractive contribution of the lipids in the beta-lipoproteins in moving-boundary electrophoresis is shown. The mobility of the main beta-component containing carbohydrate and of the transferrin is not changed by calciumion.

Submitted on June 14, 1955




The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
R. Rej
Clinical Chemistry through Clinical Chemistry: A Journal Timeline
Clin. Chem., December 1, 2004; 50(12): 2415 - 2458.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
S. B. Andersen and F. Bierring
Lymphoid Organs and Normal Gamma-Globulin in the Rat
Science, June 5, 1964; 144(3623): 1219 - 1220.
[Abstract] [PDF]

Home page
 Arch SurgHome page
O. EIKEN, D. C. NABSETH, R. F. MAYER, and R. A. DETERLING JR.
Limb Replantation: II. The Pathophysiological Effects
Arch Surg, January 1, 1964; 88(1): 54 - 65.
[Abstract] [PDF]

Home page
 Arch NeurolHome page
R. BRONNESTAM, S. J. DENCKER, and B. SWAHN
Fibrinogen in Cerebrospinal Fluid: Fibrinogen Demonstrated by Microimmunoelectrophoresis
Arch Neurol, March 1, 1961; 4(3): 288 - 290.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1956 by the American Association for Clinical Chemistry.