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Clinical Chemistry 2: 188-194, 1956;
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Clinical Chemistry, Vol 2, 188-194, Copyright © 1956 by the American Association for Clinical Chemistry

Vanadium Concentration of Urine

Rapid Colorimetric Method for Its Estimation

W. T. Rockhold 1 and N. A. Talvitie 1

1 U. S. Department of Health, Education and Welfare, U. S. Public Health Service, Occupational Health Field Headquarters, Cincinnati, Ohio.

A rapid, convenient test for the estimation of small amounts of vanadium in urine is described. The method is based on the catalytic effect of vanadium on the oxidation of N, N-diethyl-p-phenylenediamine by potassium chlorate. Only 1 ml. of urine is required and the test is sensitive to quantities of vanadium as low as 0.01 µg. per ml. of urine. Semiquantitative analyses can be made over a range of 0-1 µg. of vanadium per ml. of urine. The test is designed for application in rapid clinical analyses on small amounts of urine to determine possible vanadium absorption and is suggested for use as a screening test to determine on-the-job exposure of workers to vanadium compounds in excess of the suggested threshold limit value. Data showing the application of the test in several species are given.

Submitted on November 22, 1955






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Copyright © 1956 by the American Association for Clinical Chemistry.