Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 2: 353-368, 1956;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Carr, J. J.
Right arrow Articles by Drekter, I. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Carr, J. J.
Right arrow Articles by Drekter, I. J.

Clinical Chemistry, Vol 2, 353-368, Copyright © 1956 by the American Association for Clinical Chemistry

Simplified Rapid Technic for the Extraction and Determination of Serum Cholesterol without Saponification

Julius J. Carr 1 and I. J. Drekter 1

1 Department of Chemistry, The Mount Sinai Hospital, and the Drekter-Heisler Medical Laboratories, New York, N. Y.

An accurate yet simple procedure for the determination of total cholesterol, based upon the application of a Liebermann-Burchard color reaction directly in the solvent employed for extraction of cholesterol from serum, has been described. Extraction of cholesterol and removal of protein are accomplished by means of acetic acid and acetic anhydride. Serum water is removed by the acid-catalyzed hydrolysis of acetic anhydride. The Liebermann-Burchard color is then developed with a stable, modified reagent consisting of equal volumes of H2SO4 and acetic acid.

Excellent agreement with the technic of Schoenheimer and Sperry is obtained. Equal intensities of color are produced by equivalent concentrations of free and esterified cholesterol. Preliminary saponification of cholesterol esters is therefore not required. Color development may proceed in ordinary room lighting without loss of accuracy.

Submitted on January 14, 1956




The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
R. Rej
Clinical Chemistry through Clinical Chemistry: A Journal Timeline
Clin. Chem., December 1, 2004; 50(12): 2415 - 2458.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
R Lester, M. Carey, J. Little, L. Cooperatein, and Dowd SR
Crustacean intestinal detergent promotes sterol solubilization
Science, September 26, 1975; 189(4208): 1098 - 1100.
[Abstract] [PDF]

Home page
 ScienceHome page
H. Sobel, C. E. Mondon, and C. V. Means
Pigmy Marmoset as an Experimental Animal
Science, August 12, 1960; 132(3424): 415 - 416.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1956 by the American Association for Clinical Chemistry.