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Clinical Chemistry 20: 5-14, 1974;
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Clinical Chemistry, Vol 20, 5-14, Copyright © 1974 by the American Association for Clinical Chemistry

Principles of Competitive Binding Assays (Saturation Analyses). II. Sequential Saturation

Alfred Zettner 1 and Peggy E. Duly 1

1 Division of Clinical Pathology, Department of Pathology, School of Medicine, University of California, San Diego.

The principles of sequential saturation as a form of competitive binding assays are discussed in detail and differentiated from those of equilibrium techniques. The rationale of its applicability in practice and its potential to increase sensitivity under certain assay conditions are demonstrated with three binding systems involving digoxin, insulin, and folates. The advantages and disadvantages of the sequential saturation technique are outlined.


Key Words: radioimmunoassay • equilibrium assay technique compared with sequential saturation technique • dose-response curves • digoxin-, insulin-, and folate-binding systems • advantages and disadvantages ot the sequential saturation technique

Submitted on October 17, 1973
Accepted on November 6, 1973






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Copyright © 1974 by the American Association for Clinical Chemistry.