Clinical Chemistry
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Clinical Chemistry 20: 74-77, 1974;
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Clinical Chemistry, Vol 20, 74-77, Copyright © 1974 by the American Association for Clinical Chemistry

Identification of Hemoglobins and Hemoglobinopathies by Electrophoresis on Cellulose Acetate Plates Impregnated with Citrate Agar

Rose G. Schneider 1, Thomas S. Hosty 1, Gary Tomlin 1, and Ruby Atkins 1

1 Department of Pediatrics, University of Texas Medical Branch, Galveston, Tex. 77550; and the Bureau of Laboratories, Alabama Department of Public Health, Montgomery, Ala. 36104.

We describe a method of electrophoresis of hemoglobin on cellulose acetate plates impregnated with citrate agar, which remain stable for many months and are used to confirm rapidly the presence of hemoglobins S and C and the hemoglobinopathies associated with them. The method also provides excellent separations of hemoglobins F and A and reliable identification of genetically determined hemoglobin abnormalities in the newborn.


Key Words: inherited disorders • electrochromatography • sickle cell disease • diagnostic aid

Accepted on November 5, 1973




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W. Winter and J Yodh
Interaction of human hemoglobin and its variants with agar
Science, July 8, 1983; 221(4606): 175 - 178.
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R. B. Scott and O. Castro
Screening for Sickle Cell Hemoglobinopathies
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Copyright © 1974 by the American Association for Clinical Chemistry.