Clinical Chemistry
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Clinical Chemistry 20: 1271-1274, 1974;
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Clinical Chemistry, Vol 20, 1271-1274, Copyright © 1974 by the American Association for Clinical Chemistry

Direct Determination of Total Serum Phospholipid by X-Ray Fluorescence

Donald E. Leyden 1, Peter W. Carr 1, and William K. Nonidez 1

1 Department of Chemistry, University of Georgia, Athens, Ga. 30602.

An x-ray fluorescence method for measurement of total serum phospholipid is described. A Folch extract of 1 ml of serum is evaporated and deposited on a paper disc by washing with chloroform. A coefficient of variation of 4% was obtained for samples containing at least 1 micromole of lipid, as phosphorus. The major advantage of the method is its speed (5 min/sample) in comparison to the phosphomolybdenum blue procedure. The method is free of interference from other lipid constituents and phosphorous content can be standardized by comparison with very stable inorganic phosphates.


Key Words: spectrochemical method • heteropolyblue method compared

Submitted on May 8, 1974
Accepted on July 13, 1974






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Copyright © 1974 by the American Association for Clinical Chemistry.