Clinical Chemistry
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Clinical Chemistry 20: 1275-1281, 1974;
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Clinical Chemistry, Vol 20, 1275-1281, Copyright © 1974 by the American Association for Clinical Chemistry

Measurement of Antibodies to Insulin in Serum

K. Dixon 1

1 The General Hospital, Birmingham B4 6NH, England.

I describe a method for measuring insulin antibodies in insulin-treated subjects. The antibodies are characterized by maximum binding capacity and affinity constant. Endogenous and therapeutic insulin interferes in insulin antibody assay, and it is removed by dextran—charcoal treatment at pH 3.5. Endogenous total and free insulin may be measured by a simple extension of the method.


Key Words: maximum binding capacity • affinity constant • dextran—charcoal adsorption • measurement of total or free insulin • diabetes treatment • antibody stability • acid charcoal treatment • insulin binding curve

Submitted on May 29, 1974
Accepted on July 29, 1974




The following articles in journals at HighWire Press have cited this article:


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J. Palmer, C. Asplin, P Clemons, K Lyen, O Tatpati, P. Raghu, and T. Paquette
Insulin antibodies in insulin-dependent diabetics before insulin treatment
Science, December 23, 1983; 222(4630): 1337 - 1339.
[Abstract] [PDF]


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Copyright © 1974 by the American Association for Clinical Chemistry.