| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Clinical Chemistry, Vol 20, 1282-1286, Copyright © 1974 by the American Association for Clinical Chemistry
1 Department of Pathology, College of Medicine, University of
Iowa, Iowa City, Iowa 52242.
A previously reported direct enzymatic method for cholesterol [ Clin. Chem. 20, 470 (1974)] was critically evaluated in the clinical laboratory. Other methods compared to this enzymatic procedure were: (a) the manual Abell—Kendall procedure, giving a regression slope (proportional sensitivity) of 0.998 and a negative bias of 9.7 mg/dl in the enzymatic procedure; (b) Lipid Research Clinic procedure, giving a regression slope of 1.005 and a negative bias of 8.8 mg/dl in the enzymatic procedure; and (c) the SMA 12/60 (Technicon) method, giving a regression slope of 0.881 and a negative bias of 20.8 in the enzymatic procedure. Replicate analyses of pooled serum by the enzymatic procedure demonstrated the following inter-run precision: mean = 178 mg/dl, SD = 4 mg/dl, CV = 2.2%. Bilirubin caused a slight positive interference (1 mg of bilirubin per deciliter appears as 1-2 mg of cholesterol). Hemolysis, lipemia, and a series of steroid drugs caused no interference. A group of 465 normal subjects was tested by the enzymatic procedure. This population had a mean cholesterol of 203 mg/dl. The 2.5 percentile was 130 mg/dl, and the 97.5 percentile was 316 mg/dl.
Submitted on June 13, 1974
Accepted on July 25, 1974
The following articles in journals at HighWire Press have cited this article:
|
|
 |
|
  D. L. Rainwater, C. M. Kammerer, and J. L. VandeBerg Evidence That Multiple Genes Influence Baseline Concentrations and Diet Response of Lp(a) in Baboons Arterioscler Thromb Vasc Biol, November 1, 1999; 19(11): 2696 - 2700. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. Mahaney, J. Blangero, D. L. Rainwater, G. E. Mott, A. G. Comuzzie, J. W. MacCluer, and J. L. VandeBerg Pleiotropy and Genotype by Diet Interaction in a Baboon Model for Atherosclerosis : A Multivariate Quantitative Genetic Analysis of HDL Subfractions in Two Dietary Environments Arterioscler Thromb Vasc Biol, April 1, 1999; 19(4): 1134 - 1141. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. L. Rainwater, C. M. Kammerer, J. E. Hixson, K. D. Carey, K. S. Rice, B. Dyke, J. F. VandeBerg, S. H. Slifer, L. D. Atwood, H. C. McGill Jr, et al. Two Major Loci Control Variation in ß-Lipoprotein Cholesterol and Response to Dietary Fat and Cholesterol in Baboons Arterioscler Thromb Vasc Biol, July 1, 1998; 18(7): 1061 - 1068. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. I. Goldman, B. W. Steele, H. W. Schnaper, A. E. Fitz, E. D. Frohlich, and H. M. Perry Jr Serum Lipoprotein Levels During Chlorthalidone Therapy: A Veterans Administration-National Heart, Lung, and Blood Institute Cooperative Study on Antihypertensive Therapy: Mild Hypertension JAMA, October 10, 1980; 244(15): 1691 - 1695. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
