Clinical Chemistry
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Clinical Chemistry 20: 1287-1291, 1974;
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Clinical Chemistry, Vol 20, 1287-1291, Copyright © 1974 by the American Association for Clinical Chemistry

New Method for Determination of Aminolaevulinate Dehydratase Activity of Human Erythrocytes as an Index of Lead Exposure

Katsumaro Tomokuni 1

1 Department of Public Health, Okayama University Medical School, Okayama, Japan.

I describe a new method for measurement of aminolaevulinate dehydratase (EC 4.2.1.24) activity of human erythrocytes. In this method, the amount of substrate dgr-aminolevulinic acid consumed (instead of the amount of porphobilinogen formed) is determined colorimetrically. In the incubation mixture, the dgr-aminolevulinic acidpyrrole produced by the condensation of dgr-aminolevulinic acid with ethyl acetoacetate is separated from porphobilinogen by extraction with ethyl acetate, without resorting to ion-exchange column chromatography. The pyrrole-containing extract is treated with a modified Ehrlich's reagent. Activity of the enzyme is expressed as micromoles of dgr-aminolevulinic acid consumed per minute per liter of erythrocytes. Enzyme activity is more accurately estimated by the present method than by the usual method in which porphobilinogen is measured.


Key Words: dgr-aminolevulinic acid • colorimetry • normal values • porphobilinogen • inhibition of enzymatic activity • toxicology of lead • screening

Submitted on July 1, 1974
Accepted on July 29, 1974




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