Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 20: 1313-1319, 1974;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zettner, A.
Right arrow Articles by Duly, P. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zettner, A.
Right arrow Articles by Duly, P. E.

Clinical Chemistry, Vol 20, 1313-1319, Copyright © 1974 by the American Association for Clinical Chemistry

New Evidence for a Binding Principle Specific for Folates as a Normal Constituent of Human Serum

Alfred Zettner 1 and Peggy E. Duly 1

1 Division of Clinical Pathology, Department of Pathology, School of Medicine, the University of California, San Diego, Calif. 92103.

University Hospital, 225 West Dickinson St., San Diego, Calif. 92103.

The binding of folate by human serum was investigated by incubating serum with [3H]pteroylglutamic acid followed by charcoal separation of free and bound fractions. Sera from all of 99 normal volunteers and 9 16 patients exhibited some degree of folate binding, ranging from less than 0.1 to greater than 8 ng/ml. This binding phenomenon was found, by competitive inhibition, to be specific for folate derivatives when we used pooled serum as well as 10 individual sera representative of the entire range of folate binding as encountered in the total of 1015 sera. Studies of the association and dissociation reactions of folate and serum indicated that the binding reaction is of the reversible type. Hence, a mass-action equilibrium must also exist in vivo between bound and free folate fractions. The binding principle appears to be protein in nature. The implications of the interference of this binder in competitive binding assays of serum folates are discussed.


Key Words: competitive binding assay • radioimmunoassay

Submitted on May 20, 1974
Accepted on July 26, 1974




The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
R. Rej
Clinical Chemistry through Clinical Chemistry: A Journal Timeline
Clin. Chem., December 1, 2004; 50(12): 2415 - 2458.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1974 by the American Association for Clinical Chemistry.