Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 20: 1362-1363, 1974;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chromyacute, V.
Right arrow Articles by Kulhánek, V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chromyacute, V.
Right arrow Articles by Kulhánek, V.

Clinical Chemistry, Vol 20, 1362-1363, Copyright © 1974 by the American Association for Clinical Chemistry

Re-evaluation of EDTA-Chelated Biuret Reagent

V. Chromyacute 1, J. Fischer 1, and V. Kulhánek 1

1 Research Institute of Pure Chemicals, Lachema, N. C., 621 33 Brno; and Institute of Medical Chemistry, School of Medicine, J. E. Purkynecaron University, Brno, Czechoslovakia.

We studied the optimal composition of EDTA-chelated biuret reagent for the determination of protein in serum. A reagent containing 18 mmol of EDTA, 15 mmol of Cu2+, and 1 mol of NaOH per liter exhibits a very low blank but about the same sensitivity as tartrate-chelated reagents. Maximum color intensity is attained within 25 min at room temperature, and adheres to Beer's law up to 120 g of protein per liter.

Submitted on April 11, 1974
Accepted on July 12, 1974




The following articles in journals at HighWire Press have cited this article:


Home page
 J. Bacteriol.Home page
P. C. Dos Santos, D. C. Johnson, B. E. Ragle, M.-C. Unciuleac, and D. R. Dean
Controlled Expression of nif and isc Iron-Sulfur Protein Maturation Components Reveals Target Specificity and Limited Functional Replacement between the Two Systems
J. Bacteriol., April 1, 2007; 189(7): 2854 - 2862.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
J. M. Boyd, A. Ellsworth, and S. A. Ensign
Characterization of 2-Bromoethanesulfonate as a Selective Inhibitor of the Coenzyme M-Dependent Pathway and Enzymes of Bacterial Aliphatic Epoxide Metabolism
J. Bacteriol., December 1, 2006; 188(23): 8062 - 8069.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
D. C. Johnson, M.-C. Unciuleac, and D. R. Dean
Controlled Expression and Functional Analysis of Iron-Sulfur Cluster Biosynthetic Components within Azotobacter vinelandii
J. Bacteriol., November 1, 2006; 188(21): 7551 - 7561.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. M. Boyd, H. Ellsworth, and S. A. Ensign
Bacterial Acetone Carboxylase Is a Manganese-dependent Metalloenzyme
J. Biol. Chem., November 5, 2004; 279(45): 46644 - 46651.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. C. Dos Santos, A. D. Smith, J. Frazzon, V. L. Cash, M. K. Johnson, and D. R. Dean
Iron-Sulfur Cluster Assembly: NifU-DIRECTED ACTIVATION OF THE NITROGENASE Fe PROTEIN
J. Biol. Chem., May 7, 2004; 279(19): 19705 - 19711.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
M. K. Sluis, R. A. Larsen, J. G. Krum, R. Anderson, W. W. Metcalf, and S. A. Ensign
Biochemical, Molecular, and Genetic Analyses of the Acetone Carboxylases from Xanthobacter autotrophicus Strain Py2 and Rhodobacter capsulatus Strain B10
J. Bacteriol., June 1, 2002; 184(11): 2969 - 2977.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Christiansen, V. L. Cash, L. C. Seefeldt, and D. R. Dean
Isolation and Characterization of an Acetylene-resistant Nitrogenase
J. Biol. Chem., April 6, 2000; 275(15): 11459 - 11464.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. J. Ryle and L. C. Seefeldt
Hydrolysis of Nucleoside Triphosphates Other than ATP by Nitrogenase
J. Biol. Chem., February 25, 2000; 275(9): 6214 - 6219.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. M. Chan, M. J. Ryle, and L. C. Seefeldt
Evidence That MgATP Accelerates Primary Electron Transfer in a Clostridium pasteurianum Fe Protein-Azotobacter vinelandii MoFe Protein Nitrogenase Tight Complex
J. Biol. Chem., June 18, 1999; 274(25): 17593 - 17598.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
D. D. Clark and S. A. Ensign
Evidence for an Inducible Nucleotide-Dependent Acetone Carboxylase in Rhodococcus rhodochrous B276
J. Bacteriol., May 1, 1999; 181(9): 2752 - 2758.
[Abstract] [Full Text]

Home page
 J. Bacteriol.Home page
J. R. Allen and S. A. Ensign
Identification and Characterization of Epoxide Carboxylase Activity in Cell Extracts of Nocardia corallina B276
J. Bacteriol., April 15, 1998; 180(8): 2072 - 2078.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
J. R. Allen and S. A. Ensign
Purification to Homogeneity and Reconstitution of the Individual Components of the Epoxide Carboxylase Multiprotein Enzyme Complex from Xanthobacter Strain Py2
J. Biol. Chem., December 19, 1997; 272(51): 32121 - 32128.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. J. Small and S. A. Ensign
Alkene Monooxygenase from Xanthobacter Strain Py2. PURIFICATION AND CHARACTERIZATION OF A FOUR-COMPONENT SYSTEM CENTRAL TO THE BACTERIAL METABOLISM OF ALIPHATIC ALKENES
J. Biol. Chem., October 3, 1997; 272(40): 24913 - 24920.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. K. Sluis and S. A. Ensign
Purification and characterization of acetone carboxylase from Xanthobacter strain Py2
PNAS, August 5, 1997; 94(16): 8456 - 8461.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
W. N. Lanzilotta, K. Fisher, and L. C. Seefeldt
Evidence for Electron Transfer-dependent Formation of a Nitrogenase Iron Protein-Molybdenum-Iron Protein Tight Complex. THE ROLE OF ASPARTATE 39
J. Biol. Chem., February 14, 1997; 272(7): 4157 - 4165.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. J. Ryle, W. N. Lanzilotta, L. C. Seefeldt, R. C. Scarrow, and G. M. Jensen
Circular Dichroism and X-ray Spectroscopies of Azotobacter vinelandii Nitrogenase Iron Protein
J. Biol. Chem., January 19, 1996; 271(3): 1551 - 1557.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. J. Ryle, W. N. Lanzilotta, L. E. Mortenson, G. D. Watt, and L. C. Seefeldt
Evidence for a Central Role of Lysine 15 of Azotobacter vinelandii Nitrogenase Iron Protein in Nucleotide Binding and Protein Conformational Changes
J. Biol. Chem., June 2, 1995; 270(22): 13112 - 13117.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1974 by the American Association for Clinical Chemistry.