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Clinical Chemistry 20: 1403-1407, 1974;
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Clinical Chemistry, Vol 20, 1403-1407, Copyright © 1974 by the American Association for Clinical Chemistry

Computer Simulation and Optimization, as Exemplified by the Enzyme-Coupled Aminotransferase (Transaminase) Assays

Jack W. London 1, Robert Yarrish 1, Leonard D. Dzubow 1, and David Garfinkel 1

1 The Moore School of Electrical Engineering; The William Pepper Laboratory, Department of Pathology; and the Departments of Medicine and Biophysics of the University of Pennsylvania, Philadelphia, Pa. 19104.

We have constructed a computer model, based on experimental data and the known properties of the enzymes involved, of the aspartate aminotransferase and alanine aminotransferase assays by the coupled-enzyme procedure of Henry et al. [Amer. J. Clin. Pathol. 34, 381 (1960)] to assay sera from normal persons and persons with liver disease or myocardial infarct. Observed inhibitions of aminotransferase activity were included in the models. When the computer models are combined with an optimization procedure, substrate concentrations that result in maximum enzyme activity for individual sera are obtained. Aminotransferase activity is not much affected by rather large changes in these concentrations around the optimum. The optimal concentrations we computed are close to those currently accepted. The computer methods involved may be applied to other assays, and factors other than reagent concentration may be optimized.

Submitted on June 3, 1974
Accepted on August 8, 1974






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Copyright © 1974 by the American Association for Clinical Chemistry.