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Clinical Chemistry 20: 1441-1443, 1974;
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Clinical Chemistry, Vol 20, 1441-1443, Copyright © 1974 by the American Association for Clinical Chemistry

Comparison of Electroimmunodiffusion and Radial Immunodiffusion for Measurement of IgG in the Laboratory Diagnosis of Multiple Sclerosis

J. Joseph Perry 1, Patrick F. Bray 1, and Theodore N. Hackett Jr. 1

1 Division of Pediatric Neurology, Departments of Neurology and Pediatrics, University of Utah College of Medicine, Salt Lake City, Utah 84132.

The detection of a supranormal quantity of immunoglobulin G (IgG) in cerebrospinal fluid is valuable in diagnosis of multiple sclerosis. In a previously published study of over 300 patients, including 46 patients with a clinical diagnosis of multiple sclerosis, 74% of the patients were found to have an abnormally high IgG value, expressed as a ratio of IgG/albumin, by the reliable method of electroimmunodiffusion. In the present study the recently introduced method of radial immunodiffusion was compared with established electroimmunodiffusion technique in divided samples of cerebrospinal fluid from 49 patients with a wide range of IgG/albumin values. The correlation of the results obtained by these two methods was very good (r = 0.945), but the variance with radial immunodiffusion (±20%) is greater than with electroimmunodiffusion (±15%). Two samples that exhibited increased values by the electroimmunodiffusion method were normal with the other technique. We conclude that electroimmunodiffusion remains a slightly more accurate and sensitive technique, although radial immunodiffusion is simpler and requires less technician time.


Key Words: "kit" method • IgG/albumin ratio in CSF • normal values

Submitted on April 29, 1974






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Copyright © 1974 by the American Association for Clinical Chemistry.