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Clinical Chemistry 20: 1454-1457, 1974;
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Clinical Chemistry, Vol 20, 1454-1457, Copyright © 1974 by the American Association for Clinical Chemistry

Fully Automated, All-Enzymatic Triglyceride Method Adapted to the GEMSAEC Centrifugal Analyzer, with Use of an Aqueous Triolein Standard

R. Chong-Kit 1 and P. McLaughlin 1

1 B. C. Bio-Medical Laboratories Ltd., 7845 Edmonds St., Burnaby, B. C., Canada.

We describe a completely automated enzymatic method for estimating serum triglycerides with a centrifugal analyzer (ENI-GEMSAEC). Accuracy and precision (CV, 2 to 4.8%) and the upper limit of linearity (400 mg/100 ml) are monitored by use of newly developed, clear, aqueous triolein standards. A modified computer program (FOCAL 8) is used for data manipulation and correcting absorbance to zero reaction time. Results cornpare well with those obtained by an automated fluorometric procedure and a manual enzymatic procedure.


Key Words: "kit" method

Submitted on July 1, 1974






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Copyright © 1974 by the American Association for Clinical Chemistry.